Figure 3.

Holosphere- and merosphere-derived tumor cells generate all 3 types of tumor spheres. (A) Schematic representation of tumor sphere-derived single cells isolated from holospheres (group 1), merospheres (group 2), or paraspheres (group 3) and seeded into ultra-low adhesion culture dishes; (B) Quantification of clonogenic potential of each group of tumor cells (* p < 0.05). Note that holosphere-derived tumor cells retain higher clonogenic potential compared to merosphere- and parasphere-derived tumor cells; (C–E) Quantification of tumor spheres derived by group. Note that only holosphere- and merosphere-derived tumor cells regenerate all three types of tumor spheres. Tumor cells from paraspheres do not generate holospheres.