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. 2016 Jan 27;6:19979. doi: 10.1038/srep19979

Figure 1. Atrasentan improved renal function, decreased miR-199b-5p expression, and increased klotho expression in STZ-induced DN mice.

Figure 1

The urinary albumin/creatinine (A), serum BUN (blood urea nitrogen) (B), and serum creatinine (C) were measured to evaluate renal function. Real-time PCR was used to detect serum and renal tubular epithelial cell miR-199-5p expression (D). The serum klotho level was measured with an ELISA kit, and the renal klotho level was detected with real-time PCR and a Western blot (E). The levels of urinary KIM-1(kidney injury molecule-1) (F), NGAL (neutrophil gelatinase-associated lipocalin) (G) and NAG (N-acetyl-β-D glucosminidase) (H) were measured by ELISA kits. A Caspase-3 activity assay (F) and a TUNEL assay (G) were used to assess the apoptosis of renal tubular epithelial cells of mice. n = 10. *P < 0.05 versus the control group; #P < 0.05 versus the STZ-induced DN group.