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. 2016 Jan 27;6:19979. doi: 10.1038/srep19979

Figure 6. miR-199b-5p participated in the effects of atrasentan on the expression of klotho, antioxidant activities, and caspase activity of HK-2 cells.

Figure 6

The HK-2 cells were treated with 20 mmol/L glucose and co-incubated with atrasentan. An miR-199b-5p mimic (100 mmol) or an NC was then transfected into the cells. The concentration of klotho treated in cells was 20 nmol/L. The expression of klotho was measured by real-time PCR and a Western blot (A). Levels of antioxidant indicators (T-AOC, SOD, CAT, and GSH) of renal mitochondria were detected by commercial kits in the HK-2 cells (B–E). The activity of caspase-3 was detected with an ELISA kit (F). *P < 0.05 versus cells treated with 20 mmol/L glucose; #P < 0.05 versus the cells treated with 20 mmol/L glucose + atrasentan + NC; $P < 0.05 versus the cells treated with20 mmol/L glucose + atrasentan + miR-199b-5p mimic + PBS.