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. 2015 Dec 25;8(1):6. doi: 10.3390/toxins8010006

Figure 2.

Figure 2

Activity of BdCXEs expressed in yeast strain YZGA2274 (ayt1 pdr5,10,15 rps11a) (a) expressing the indicated genes spotted in three dilutions on synthetic complete medium (SC) plates lacking uracil and containing the indicated amounts of toxin; (b) hydrolysis of 12 mg/L 3-acetyl-deoxynivalenol (3-ADON) into deoxynivalenol (DON) and (c) 15-acetyl-deoxynivalneol (15-ADON, 12 mg/L) to DON and the release into the supernatant by protein extracts of transformed yeast expressing BdCXE29, BdCXE52 and Tri8 from a 15-ADON chemotype Fusarium graminearum strain (Tri8-15AD) or a 3-ADON chemotype strain (Tri8-3AD).