Exposure time, turkey type, and gender cause variation in embryonic transcriptomes. Principle component analysis (PCA) was performed on regularized log2 transformed read counts in DESeq2 [30]. Principle component 1 (PC1) and principle component 2 (PC2) explain 42% of the variation in read counts. Samples are plotted by group: DTC1 (blue circles), DTC5 (dark blue diamonds), DTA1 (green pentagons), DTA5 (dark green inverted triangles), WTC1 (red squares), WTC5 (dark red triangles), WTA1 (gold hexagons), and WTA5 (dark gold crosses). Gender for each sample is also indicated (male = solid, female = striped). Domesticated turkey (DT), wild turkey (WT), control 1 day (C1), control 5 days (C5), aflatoxin B1 1 day (A1), aflatoxin B1 5 days (A5).