Table 3. Identification of the cytochrome c oxidases by ESI-Q-ToF mass spectrometry in solubilised membranes of S. oneidensis MR-1 and the cbb ₃ oxidase deletion strain (SLL01) grown under carbon-depleted conditions (MM3C⁻).

O2 condition	Strain	Growth phasea	Bandb	Protein namec	NCBI enter	Gene	Peptd	Cove
Aerobic conditions	S. oneidensis Wild type	E	I	No cytochrome c oxidase detected
			II	cbb3 cyt c oxidase sub. II	24348335	ccoO	23	47
				cbb3 cyt c oxidase sub. III	24373908	ccoP	11	40
		S	I	No cytochrome c oxidase detected
			II	cbb3 cyt c oxidase sub. II	24348335	ccoO	17	39
				cbb3 cyt c oxidase sub. III	24373908	ccoP	27	65
	S. oneidensis SLL01	E	III	No cytochrome c oxidase detected
		S	III	No cytochrome c oxidase detected
			IV	A-type cyt c oxidase sub. II	24376079	coxB	17	38
			V	A-type cyt c oxidase sub. II	24376079	coxB	8	22
Highly aerobic conditions	S. oneidensis Wild type	E	VI	No cytochrome c oxidase detected
			VIII	cbb3 cyt c oxidase sub. II	24348335	ccoO	26	53
				cbb3 cyt c oxidase sub. III	24373908	ccoP	13	58
		S	VI	No cytochrome c oxidase detected
			VII	cbb3 cyt c oxidase sub. II	24348335	ccoO	17	35
				A-type cyt c oxidase sub. II	24376079	coxB	11	25
			VIII	cbb3 cyt c oxidase sub. II	24348335	ccoO	25	54
				A-type cyt c oxidase sub. II	24376079	coxB	8	15
	S. oneidensis SLL01	S	IX	A-type cyt c oxidase sub. II	24376079	coxB	12	22
			X	No cytochrome c oxidase detected

^aExponential (E) or stationary (S) phase of growth.

^bRoman numerals refer to the protein bands from the BN gel shown in Fig. 3.

^cProtein name in NCBI database. Cyt: cytochrome. Sub.: subunit.

^dNumber of peptides detected.

^eProtein sequence coverage by the matching peptides (%).

These results are representative of three similar experiments.