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. 2015 Dec 24;8(1):4. doi: 10.3390/v8010004

Figure 2.

Figure 2

Scheme of recombinant S1 gene segment compared to wild-type T3DS1. rS1-2A-yTG is the recombinant S1 segment containing your transgene (yTG) downstream of a porcine teschovirus-1 element (P2A) to separate the encoded protein from the σ1 part. Entry of the recombinant reovirus by sialic acids is provided by a mutation in the S1-part that results in a G to R amino acid change at position 196 (G196R) in the truncated σ1 protein. In the 3’Untranslated region (UTR) of the recombinant gene the C-box is important for incorporation of the segment in the viral particles [46]. To not exceed the wild-type size of S1, the inserted transgene might be ~453 nt long. The size of each element is depicted in nucleotides (nt) below each segment.