Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 25;8(1):6. doi: 10.3390/v8010006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Protein-binding assays in biological liquids using microscale thermophoresis (MST). (A) The binding curve of quercetin with NT647 labeled recombinant influenza HA protein; (B) The binding curve of CL-385319 with NT647 labeled recombinant influenza HA protein. NT647 (NanoTemper Technologies) labeled recombinant influenza HA protein was mixed with two-fold diluted compounds in the same volume as that of the final concentrations. MST experiments were performed on a Monolith NT.115 system (NanoTemper Technologies) using 100% LED and 20% IR-laser power. The compound CL-385319 was used as the positive control. K_d means the dissociation constant.

Protein-binding assays in biological liquids using microscale thermophoresis (MST). (A) The binding curve of quercetin with NT647 labeled recombinant influenza HA protein; (B) The binding curve of CL-385319 with NT647 labeled recombinant influenza HA protein. NT647 (NanoTemper Technologies) labeled recombinant influenza HA protein was mixed with two-fold diluted compounds in the same volume as that of the final concentrations. MST experiments were performed on a Monolith NT.115 system (NanoTemper Technologies) using 100% LED and 20% IR-laser power. The compound CL-385319 was used as the positive control. K_d means the dissociation constant.