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. 2015 Dec 29;8(1):8. doi: 10.3390/v8010008

Figure 2.

Figure 2

TNKS degradation is not inhibited upon HCMV infection. (A) Western blot showing ubiquitination of TNKS immunoprecipitated from non-infected and HCMV-infected HFFs using an anti-ubiquitin antibody. HFFs were infected with HCMV Towne at MOI = 1 and harvested at 72 hpi; (B) Western blot showing stability of TNKS after 48 h of infection at MOI = 3 followed by treatment with cycloheximide to inhibit de novo translation and harvested at indicated time points. M indicates treatment with MG132 as a proteasome inhibitor control; (C) Quantification of TNKS’ degradation normalized to β-actin from (B) Cycloheximide was added at 48 hpi for the times indicated in the X axis (h of CHX). Data shown are the average of three experiments with standard error of the means.