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. 2015 Dec 29;8(1):8. doi: 10.3390/v8010008

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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HCMV inactivates TNKS activity. (A) Western blot showing auto-PARsylation activity of immunoprecipitated TNKS at 72 hpi with HCMV Towne at MOI = 3. Immunoprecipitated TNKS was used for the PARsylation assay as described in Materials and Methods. XAV939 was included as a positive control for TNKS inhibition; (B) Proteasomal degradation assay from lysates assayed in (A) to indicate lysate quality and activity; (C) Western blot showing detection of PAR residues on TNKS immunoprecipitated from non-infected and HCMV-infected HFFs (MOI = 3 at 72 hpi); (D) Detection of PAR residues by Western blot in non-infected and HCMV Towne-infected HFF (MOI = 3, 72 hpi) cell lysates to show no loss of PARsylation activity from other PARPs in general; (E) Western blot showing detection of PAR residues on Axin1 immunoprecipitated from non-infected and HFFs infected by HCMV Towne at MOI = 3 at 72 hpi.

HCMV inactivates TNKS activity. (A) Western blot showing auto-PARsylation activity of immunoprecipitated TNKS at 72 hpi with HCMV Towne at MOI = 3. Immunoprecipitated TNKS was used for the PARsylation assay as described in Materials and Methods. XAV939 was included as a positive control for TNKS inhibition; (B) Proteasomal degradation assay from lysates assayed in (A) to indicate lysate quality and activity; (C) Western blot showing detection of PAR residues on TNKS immunoprecipitated from non-infected and HCMV-infected HFFs (MOI = 3 at 72 hpi); (D) Detection of PAR residues by Western blot in non-infected and HCMV Towne-infected HFF (MOI = 3, 72 hpi) cell lysates to show no loss of PARsylation activity from other PARPs in general; (E) Western blot showing detection of PAR residues on Axin1 immunoprecipitated from non-infected and HFFs infected by HCMV Towne at MOI = 3 at 72 hpi.