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. 2015 Dec 29;8(1):8. doi: 10.3390/v8010008

Figure 4.

Figure 4

TNKS inhibition favors HCMV replication. (A) Plaque assay for HCMV Towne and TB40 strains with XAV939 (PARP5 inhibitor), and ABT-888 (PARP1and 2 inhibitor). HFFs were infected to yield approximately 50-100 plaques/well, stained and quantified at 10 days after plating; (B) Western blot to detect viral proteins at 72 hpi in HFFs infected with HCMV Towne at MOI = 1. Fold change in protein expression normalized to β-actin are quantified under the blot; (C) Plaques from (A) imaged after staining at 10× magnification; (D) Viral growth assay for Towne and TB40 strains with XAV939 (PARP5 inhibitor), Salinomycin (Wnt inhibitor) and ABT-888 (PARP1and 2 inhibitor). HFFs were infected and supernatants were collected at 24-h intervals till 120 hpi. Released virions were titered by a standard plaque assay. Average pfu/mL calculated from quadruplicate wells of one experiment are shown. * indicates p value < 0.05, ** indicates p < 0.01.