Table 1.
Efficiency of plating (EOP) of bacteriophages and their mutants on different E. coli host strains. The strain 4sR ltfAR is a mutant of E. coli 4sR selected for resistance for DT571/2 ltfA am phage mutant (two independent clones having different mutations in btuB gene had the same phenotype so this strain designation stays for both of them).
| Bacteriophage Strains | Bacterial Host Strains | |||||||
|---|---|---|---|---|---|---|---|---|
| 4s | 4sR | 4sltfAR ltfAR | 4sI | 4sI:pwclK | HS1/2 | C-600 | 3/104 | |
| BF23 | − | + | − | + | − | − | + | − |
| DT571/2 | − | + | − | + | − | + | + | + |
| DT5712 ltfA am | − | + | − | + | − | − | + | − |
| DT5712 ltfB opal | − | + | − | + | − | 10−1b | + | 10−5a |
| DT571/2-ABF | − | + | − | + | − | − | + | + |
| DT57C | + | + | − | + | + | − | + | + |
| DT57C(4s-) | I | + | − | + | I | − | + | I |
| DT530 | + | + | − | + | + | − | + | + |
| DT530(1) | − | + | − | + | − | − | + | I |
“+“—Efficiency of plating (EOP) >0.5; “−“—No phage growth visible in the spot; “I”—No plaques formed, but the growth inhibition zone appears if a drop of the concentrated phage stock is applied onto the lawn; a EOP = 10−5, normal plaques formed; b In fresh lysate EOP of ca. 10−1 was observed that dropped after the phage purification. Plaques are very small and turbid.