Fig. 4.

Increased TLR4 expression by fibroblast after MIF stimulation. a Mouse fibroblast cells were stimulated with two different concentrations of MIF for 48 h. Transcription levels of the TLR4 gene in mouse fibroblasts were up-regulated by about 33 % after treatment with MIF at 375 ng/ml. **P < 0.01 (b) The above finding was further verified by flow cytometry. All cells were stained by PE dye, and 375 ng/ml MIF-treated samples showed clear curve shift to the right of the PE-signal (red peak vs black peak); 20 % elevation of TLR4 in fibroblast cells was observed. Data are representative of 3 independent experiments