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. 2016 Jan 26;17:43. doi: 10.1186/s12891-016-0895-0

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© Xi et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Co-stimulatory effect of MIF on LPS-induced fibroblast proliferation. LPS was used at 25 μg / ml, alongside several MIF concentrations, including 375 ng/ml, 187 ng/ml and 93 ng/ml. In presence of LPS, OD450 value increased with MIF dose, which indicated MIF could co-stimulate fibroblast proliferation. Without LPS, MIF could not stimulate fibroblast proliferation within the dose range tested. This indicated that stimulatory effect of MIF on fibroblast proliferation was indirect, requiring LPS