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. 2016 Jan 27;35:19. doi: 10.1186/s13046-016-0296-0

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Smad4 is associated with miR-130a-3p expression. a Top panel: Real-time RT-PCR assay was performed to detect the mRNA level of Smad4 in HCC GR cells treated with miR-130a-3p mimics. miR-130a-3p was measured by miRNA real-time RT-PCR in HCC GR cells after miR-130a-3p mimic transfection. Bottom panel: Real-time RT-PCR assay was performed to detect the mRNA level of Smad4 in HCC cells treated with miR-130a-3p inhibitor. miR-130a-3p was measured by miRNA real-time RT-PCR in HCC cells after miR-130a-3p inhibitor treatment. *p < 0.05, vs control. b Left panel: Western blotting analysis was conducted to measure the expression of Smad4 in HCC cells treated with miR-130a-3p inhibitor and in HCC GR cells treated with miR-130a-3p mimic. Right panel: Quantitative results are illustrated for left panel