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. 2016 Jan 27;35:19. doi: 10.1186/s13046-016-0296-0

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Down-regulation of Smad4 reverses EMT to MET in HCC GR cells. a Left panel: Real-time RT-PCR assay was used to detect the mRNA level of Smad4 in HepG2 GR cells transfected with Smad4 siRNAs. Right panel: Western blotting analysis was performed to measure the expression of Smad4 in HepG2 GR cells after Smad4 siRNA transfection. b Down-regulation of Smad4 caused reversal of EMT phenotype of HCC GR cells. HepG2 GR and SMMC-7721 GR cells transfected with control siRNA exhibited a fibroblastic-type phenotype, while these GR cells transfected with Smad4 siRNA display round-like epithelial cell shape. c HCC GR cells transfected with control siRNA or Smad4 siRNA were used for assessing the expression of EMT markers using Real-time RT-PCR. * P < 0.05, ** P < 0.01 vs control. d HCC GR cells transfected with control siRNA or Smad4 siRNA were used for assessing the expression of EMT markers by Western blotting analysis. CS: control siRNA; SS: Smad4 siRNA