Fig. 1.

Identification of LL motifs which regulate ASIC2a expression and surface trafficking. a Diagram showing the C-terminus of WT ASIC2a and the mutants generated. The second transmembrane domain is highlighted by gray shading. b Blots showing the specificity of the ASIC2 antibody. WT and ASIC2−/− (2KO) brain lysate was blotted with the ASIC2 antibody. Note that the specificity of this antibody has been verified in a recent study [17] but we presented here a similar blot for the clarity of this study. c-e Representative western blot and quantification showing the effect of the corresponding mutants on expression and surface trafficking of ASIC2a. CHO cells were transfected with wild-type or mutant ASIC2a as indicated. Surface proteins were labeled by surface biotinylation and isolated by NeutrAvidin pulldown. Surface and total proteins were analyzed by Western blot. Numbers on the bars indicate the total number of repeats. p values are from one-tailed t-test