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. 2016 Jan 27;9:9. doi: 10.1186/s13041-016-0190-x

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© Wu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effect of LL mutants on subcellular localization in CHO cells. CHO cells were transfected with HA-ASIC2a WT (a), AAD (b) DAA (c) or HA-ASIC1a (d) together with eGFP. ASIC localization was revealed by anti-HA immunofluorescence and visualized with confocal microscopy. Note that compared to ASIC1a (D), ASIC2a shows a more membranous localization