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. 2016 Jan 27;9:9. doi: 10.1186/s13041-016-0190-x

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© Wu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effect of LL mutants on N-glycosylation of ASIC2a. Representative Western blots showing the effect of AADAA (a), AAD and DAA (b) on N-glycosylation status of ASIC2a. CHO cells were transfected with wild-type or mutant ASIC2a as indicated. Total cell lysates were untreated (U), treated with PNGase F (P), or treated with Endo H (E), and analyzed by Western blot. Numbers on the bar indicate total number of repeats. The p value was from paired t-test in (a) and Anova followed by Turkey’s HSD test in (b)