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Genetic confirmation of LNCaP origin by DNA sequencing. To determine the origin of the JHU-LNCaP-SM cell line, genomic DNA sequencing analysis was performed on segments with known LNCaP AR mutations. The sequencing showed that the LNCaP, JHU-LNCaP-SM, and C4-2 lines all contained the LNCaP specific AR mutation (ACT–GCT).
