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. Author manuscript; available in PMC: 2016 Feb 1.
Published in final edited form as: Prostate. 2015 Oct 26;76(2):215–225. doi: 10.1002/pros.23115

Fig. 8.

Fig. 8

PSMA-expressing JHU-LNCaP-SM subcutaneous xenografts spontaneously metastasize to lymph nodes. (A) Ex vivo PSMA-targeted optical imaging of lymph nodes from selected mice that exhibited spontaneous metastasis from subcutaneous JHU-LNCaP-SM primary xenografts where green arrows show PSMA positive axillary lymph nodes and red is a negative node. (B and C) The red circle is a positive renal lymph node and also shown is a superficial cervical lymph node denoted by a green arrow. (D) Frozen sections of lymph node metastasis were analyzed by immunofluorescence for PSMA and CD31 expression and confirm the presence of PSMA-expressing cells within a vascular cage at the edge of packed lymphatic cells (scale bar = 100 μm). (E–H) H&E staining of various PSMA positive nodes confirming the presence of tumor cells (scale bar = 50μm).