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. Author manuscript; available in PMC: 2017 Feb 3.
Published in final edited form as: J Ethnopharmacol. 2015 Dec 9;178:307–322. doi: 10.1016/j.jep.2015.12.006

Figure 1.

Figure 1

SEAP reporter activity of P. tuerckheimii (PT), P. jacquemontianum (Pag) and Cissampelos tropaeofolia (CT). Methanol extracts of P. aeruginosibaccum (Pag) was estrogenic alone and also enhanced the SEAP activity of E2. P. tuerckheimii (PT) was not estrogenic alone, but interestingly, strongly enhance the activity of E2 in MCF-7 cells. C. tropaeolifolia DC. (CT, 20 μg/ml) was weakly estrogenic alone and also enhanced the SEAP activity of E2 in MCF-7 cells. Secreted embryonic alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression. It is a truncated form of human placental alkaline phosphatase (PLAP) by deletion of the GPI anchor. SEAP supplies and kits were obtained from InVivoGen (San Diego, CA) and performed as described by the manufacturer and methods were as previously described (Patel et al., 2014; Cullen et al., 1995).