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. Author manuscript; available in PMC: 2017 Feb 3.
Published in final edited form as: J Ethnopharmacol. 2015 Dec 9;178:307–322. doi: 10.1016/j.jep.2015.12.006

Figure 4.

Figure 4

In vitro SEAP reporter gene activity of Brosimum alicastrum. Methanol extracts of the fruit pulp and seeds (BAPS, 20 μg/ml) were estrogenic alone and enhanced the SEAP activity in MCF-7 breast cancer cells. The activity of BAPS was shown to be ER dependent as when MCF-7 cells were treated with BAPS and ICI 182780 (an ER antagonist that promotes degradation of the ER), the activity of the extract was significantly reduced demonstrating that the ER was necessary for this activity. Results and methods were previously reported (Cullen et al., 1995; Patel et al., 2014).