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. 2016 Feb;186(2):248–258. doi: 10.1016/j.ajpath.2015.10.001

Figure 6.

Figure 6

Analysis of the density of the glycocalyx in cultured endothelial cells exposed for 10 minutes to LPS with and without treatments. A: An analyzed cell from each group is shown on the left, adjacent to its respective image obtained with the use of the 2D Circle packing algorithm of ImageJ. The algorithm created space-filling ellipses and circles to highlight areas depleted of glycocalyx. B and C: The distribution plots in B and the box and whisker plots in C show a statistically significant difference between the mean of the control group and the means of the three experimental groups. In both B and C, the mean radius of NOHA and EPC-CM are similar and are statistically smaller than that of LPS, representing smaller lesions in the two treatments. n = 40 to 50. *P < 0.05 versus control, †P < 0.05 versus LPS. Original magnification, ×63. CM, conditioned medium; EPC, endothelial progenitor cell; LPS, lipopolysaccharide; NOHA, NG-hydroxy-l-arginine; 2D, two-dimensional.