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. 2016 Feb;186(2):347–358. doi: 10.1016/j.ajpath.2015.10.002

Figure 2.

Figure 2

Tg26 mice display higher renal tissue mRNA expression of NLRP3, caspase-1, ASC, and IL-1β. Total RNA was extracted from renal tissue of FVBN and Tg26 mice. A–D: RNA was converted into cDNA with the use of random hexamer primers. Fold-expression analysis of NLRP3 (A), caspase-1 (B), ASC (C), and IL-1β (D) mRNA expression were quantified by quantitative real-time PCR with the use of specific universal probe numbers and primers of each mRNA. Data are expressed as dot plots from each sample. Mean values are represented as horizontal bar. n = 6 FVBN mice; n = 6 Tg26 mice. ∗∗∗P < 0.001 versus respective FVBN. ASC, apoptosis-associated speck-like protein containing CARD and PYD domains; NLRP, nucleotide oligomerization domain (Nod)-like receptor protein; Tg26, HIV-transgenic.