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. 2016 Feb;186(2):347–358. doi: 10.1016/j.ajpath.2015.10.002

Figure 5.

Figure 5

HIV activates NLRP3 inflammasome in podocytes. A: Protein blots of control and V/HPs were probed for caspase-1 expression, and the same blots were stripped and re-probed for actin. Representative gels are displayed. B: Densitometric data of caspase-1 expression (control versus V/HPs) are shown as dot plot. No difference was found between control and V/HPs in NLRP3 expression. C: Protein blots of EV/HPs and HIV/HPs were probed for NLRP3, Casp1 (catalog no. sc-560360; Santa Cruz Biotechnology), IL-1β (31.0 kDa), cleaved IL-1β (17.0 kDa), and actin. Representative gels are displayed. D–G: Cumulative densitometric data of NLRP3 (D), Casp1 (E), IL-1β (F), and cleaved IL-1β (G) expression are shown as dot plots. H: To check cleaved Casp1, protein blots from thee different sets of V/HPs and HIV/HPs were probed with Casp1 antibody (catalog no. 4199; cleaved Casp1 no. Asp297/D57A2; Cell Signaling) and reprobed for actin. I and J: Representative gels are displayed. Cumulative densitometric data (Casp1, I; p20, J) are shown as dot plot. Data are expressed as means (horizontal bar) ± SD (scatter). n = 3 (A, C, and H). ∗P < 0.05 versus control; ∗∗∗P < 0.001 versus HIV. C, control; Casp1, caspase-1; HP, HIV expression by podocyte; NLRP, nucleotide oligomerization domain (Nod)-like receptor protein; V, vector.