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. 2016 Feb;186(2):347–358. doi: 10.1016/j.ajpath.2015.10.002

Figure 6.

Figure 6

Casp1Inh reduces the activation of HIV-induced inflammasome in podocytes. V/HPs and HIV/HPs in the presence of absence of 5 μmol/L Casp1Inhfor 48 hours (n = 3). Cell lysates were prepared and protein blots were probed for Casp1 (catalog no. sc-560360; Santa Cruz Biotechnology). A: The same blot was stripped and re-probed for IL-1β and actin. Representative gels are displayed. B: Induction of Casp1 expression in response to HIV and inhibition with Casp1Inhis represented as dot plot. C: Processing of IL-1β in response with HIV and inhibition in the presence of Casp1Inhis represented as dot plot. D: To analyze cleaved Casp1 p20, protein blots of samples treated with V, HIV, and HIV Casp1Inh were probed with Casp1 antibody (catalog no. 4199; cleaved Casp1 Asp297/D57A2; Cell Signaling). Representative gels are displayed. E and F: Cumulative densitometric data pertaining to intact Casp1expression (E) and cleaved Casp1 (F) are shown as dot plots. Data are expressed as means (horizontal bar) ± SD (scatter). n = 3. ∗P < 0.05 versus respective HIV; ∗∗P < 0.01 versus respective HIV; ∗∗∗P < 0.001 versus V. Casp1, caspase-1; Casp1Inh, caspase-1 inhibitor; HP, HIV expression by podocyte; V, vector.