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. 2015 Dec;185(12):3211–3223. doi: 10.1016/j.ajpath.2015.08.004

Figure 1.

Figure 1

Repression of Klotho is associated with renal renin-angiotensin system (RAS) activation in a mouse remnant kidney model. A: Western blot analyses of renal expression of Klotho and RAS components in sham control and 5/6 nephrectomized mice (5/6NX). Kidney lysates from sham control and 5/6NX mice at 6 weeks as indicated were immunoblotted with antibodies against Klotho, AGT, renin, AT1, and actin, respectively. B–E: Graphic presentation of the relative abundance of Klotho (B), AGT (C), renin (D) and AT1 (E) in two groups as indicated. F: Representative micrographs show Klotho, AGT, renin, ACE, and AT1 protein expression in two groups. Paraffin-embedded kidney sections were stained with different antibodies. Arrows indicate positive staining. ∗P < 0.05 versus sham controls. n = 5 to 6 (B–E). Scale bar = 50 μm (F).