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. 2015 Dec;185(12):3211–3223. doi: 10.1016/j.ajpath.2015.08.004

Figure 4.

Figure 4

Expression of secreted Klotho in vivo abolishes renin-angiotensin system (RAS) induction in mouse remnant kidney model. A: Representative micrographs show Klotho and RAS components in different groups as indicated. Kidney sections were stained with different antibodies against Klotho, AGT, renin, ACE, and AT1. Arrows indicate positive staining. B: Representative Western blot analyses show membranous and secreted Klotho in kidney lysates. Renal endogenous membranous Klotho (mKlotho) and secreted Klotho (sKlotho) were indicated. Quantitative analyses demonstrate the levels of renal mKlotho (C) and sKlotho (D) at 6 weeks in 5/6NX mice (7 days after last injection of pV5-sKlotho plasmid). E: Representative Western blot analyses reveal that Klotho abolishes RAS induction in 5/6NX mice. Kidney lysates from different groups as indicated were immunoblotted with antibodies against AGT, renin, ACE, AT1, and actin, respectively. Numbers (1 to 5) represent different animals in a given group. F–I: Graphic presentations of the relative abundances of AGT (F), renin (G), ACE (H), and AT1 (I) in different groups as indicated. ∗P < 0.05 versus 5/6NX alone. Scale bar = 50 μm (A). 5/6NX, 5/6 nephrectomized mice injected with pcDNA3; 5/6NX+K, 5/6 nephrectomized mice injected with pV5-sKlotho.