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. 2015 Dec;185(12):3141–3151. doi: 10.1016/j.ajpath.2015.08.020

Figure 3.

Figure 3

Deletion of miR-223 enhances insulin-like growth factor 1 receptor (IGF1R) signaling during Fas-induced liver injury. Wild-type (WT) and miR-223 knockout (KO) mice were i.p. injected with 0.5 μg/g body weight Jo2. In silico analysis for target gene prediction using TargetScan led to identification of Igf1r as a candidate gene of miR-223 target. Western blot analysis was performed to measure the levels of IGF1R and p-IGF1R as well as its downstream target Akt. The miR-223 KO livers show higher levels of IGF1R, p-IGF1R, and p-Akt compared with WT livers after Jo2 treatment. Representative Western blots and the average densitometry data are shown. Data indicate means ± SD. N = 6 per group. ∗P < 0.05.