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. 2015 Dec;185(12):3141–3151. doi: 10.1016/j.ajpath.2015.08.020

Figure 4.

Figure 4

The effect of insulin-like growth factor 1 receptor (IGF1R) inhibitor on Fas-induced liver injury. Wild-type (WT) and miR-223 knockout (KO) mice were injected with 25 mg/kg of body weight IGF1R inhibitor (NVP-AEW541) or vehicle [dimethyl sulfoxide (DMSO)] 30 minutes before injection with 0.5 μg/g body weight Jo2 (the animals were sacrificed 4 hours after Jo2 injection. A: The levels of serum transaminases [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)]. B: Representative hematoxylin and eosin staining of the liver tissues. C: Caspase-3 immunostaining of the liver tissues. The percentages of caspase-3–positive cells are shown. D: Caspase-3, caspase-8, and caspase-9 activities in the liver tissues. The data are expressed as means ± SD (A) or means ± SD of fold changes (D). N = 6 per group (A); N = 3 (D). ∗P < 0.05, ∗∗P < 0.01. Original magnification, ×100 (B and C).