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. 2015 Dec;185(12):3141–3151. doi: 10.1016/j.ajpath.2015.08.020

Figure 5.

Figure 5

Insulin-like growth factor 1 receptor (IGF1R) is a direct target of miR-223. A: Putative miR-223 binding site in IGF1R–3′-untranslated region (UTR). The IGF1R–3′-UTR sequence that contains seven base nucleotides interacting with miR-223 and the sequence of IGF1R–3′-UTR-mutant generated in this study (with mutation of two nucleotides in the seed sequence) are shown. B: IGF1R 3′-UTR luciferase reporter activity assay. Primary hepatocytes isolated from wild-type (WT) mice were cotransfected with miR-223 mimic plus IGF1R 3′-UTR reporter plasmid (with or without miR-223 binding site mutation). miR-223 mimic decreases the IGF1R 3′-UTR luciferase activity when the cells were transfected with the WT IGF1R 3′-UTR reporter construct; this effect is abolished when the miR-223 binding site at the IGF1R 3′-UTR is mutated. ∗∗P < 0.01.