Table 2.

Summary of one mtDNA and four nDNA loci and their amplification conditions used for phylogenetic analysis of 86 tortoises in the genus Gopherus

Locus	# of haplotypes	Length (bp)	# variable sites	MgCl2 (mmol/L)	Annealing temp °C	Citation
mtDNA (ND3/ND4)	18	1109	190	4	52	Edwards 2003; Edwards et al. (2014)
BDNF	6	640	5	2	57	Leaché and McGuire (2006)
R35	13	500	17	4	53.4	Spinks et al. (2004)a
TB02	16	425	13	1	59	Thomson et al. (2008)
TB07	13	590	10	1.5	58	Thomson et al. (2008)

^aOriginal primers from Fujita et al. (2004).