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. Author manuscript; available in PMC: 2016 Jan 27.
Published in final edited form as: Expert Rev Mol Diagn. 2004 Sep;4(5):663–676. doi: 10.1586/14737159.4.5.663

Figure 3. Schematic representation of spectral karyotyping hybridization experimental set up.

Figure 3

(A) Flow sorting is used to isolate individual chromosomes. (B) Each chromosome is then labeled with a unique combination of fluorescent dyes or haptens. (C) The hybridization cocktail (a combination of these differentially labeled chromosome painting probes and Cot-I DNA to suppress the hybridization of repeat sequences) is hybridized to tumor metaphase preparations. (D) After washes and detection of the hapten labeled probes, the metaphases are imaged using a fluorescent microscope. (E) A spectrophorometer in the imaging system generates a spectral signature for each pixel in the image. (F) Each pixel is assigned a classification color based on its spectral signature. Each normal chromosome is therefore a single color.