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Further assessment of soluble expression of GST-hD2 by applying affinity purification step to the soluble fraction prepared from osmotic shocks (0.5 M NaCl and 1mM glycinebetaine) (extension to experiment shown in panel A of Figure 10). The digital part of the alphanumerical codes above the lanes show incubation times after induction. (S) and (I) symbols of the code denote soluble and insoluble fractions, respectively. P and F represent respectively, purified GST-hD2 and flowthrough.
