Figure 3.

Further assessment of soluble expression of GST-hD2 by applying affinity purification step to the soluble fraction prepared from the culture grown at 10 °C and induced by 0.3 mM lactose (extension to experiment shown in panel D of Figure 1). The digital part of the alphanumerical codes above the lanes show incubation times after induction. (S) and (I) symbols of the code denote soluble and insoluble fractions, respectively. L, P and F represent respectively a 50 kDa ladder protein, purified GST-hd2 and flowthrough.