Figure 6.
Effect of shakingon soluble expression of GST-hD2. The digital part of the alphanumerical codes above the lanes show incubation times after induction. (S) and (I) symbols of the code denote soluble and insoluble fractions, respectively. A Subculture in absence of inducer and cultivated at 37 °C, 200 rpm for 4 h then, the shaking speed was decreased to 50 rpm. B starter culture and subculture were grown at 30 °C, 120 rpm. The subculture was induced by 0.1 mM IPTG and the cultivation condition was changed to 30 rpm at 25 °C.