Figure 7.
Effect of Mg2+on soluble expression of GST-hD2. The cells were induced by 0.6 mMlactose at 20 °C. The digital part of the alphanumerical codes above the lanes show incubation times after induction. (S) and (I) symbols of the code denote soluble and insoluble fractions, respectively. A Magnesium chloride was used at 0.1 and 10 M concentrations. B Further assessment of soluble expression of GST-hD2 by applying affinity purification step to the soluble fraction prepared from the culture grown at 20 °C and induced by 0.6 mMlactose in the presence of magnesium chloride in 10 mM final concentration. P and F represent purified GST-hD2 and Flowthrough respectively.