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. Author manuscript; available in PMC: 2016 Jan 27.
Published in final edited form as: J Biol Chem. 1988 Nov 25;263(33):17390–17396.

Fig. 3. Regulated expression of a rat AGP-CAT gene construct in HepG2 cells.

Fig. 3

HepG2 cells in 6-well cluster plates were transfected with plasmid DNA mixture (18 μg of pAGP(3xDRE)-140-CAT and 1 μg of pIE-MUP/ml). After a recovery period of 24 h and culture in serum-free MEM for 16 h, the cells were treated for 8 h with MEM containing the indicated factors (0.15 μM TPA, 100 units/ml IL-6, 250 units/ml IL-1α; 0.1 μM dexamethasone (Dex)). The thin layer chromatography pattern of the chloramphenicol acetyltransferase (CAT) activity in 10 μl of cell extract after 20 h autoradiography is reproduced. The rocket immunoelectrophoresis of the MUP secreted by the same cells during the final 24 h period is shown at the top. The specific chloramphenicol acetyltransferase activity was determined by using 0.3–30 μl of the cell extract and the values were related to the amount of MUP produced.