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. Author manuscript; available in PMC: 2016 Jan 27.
Published in final edited form as: J Biol Chem. 1988 Nov 25;263(33):17390–17396.

Fig. 5. Binding of IL-1 and IL-6 to HepG2 cells.

Fig. 5

Confluent monolayers of HepG2 cells were pretreated for 24 h with serum-free MEM alone (○), or with MEM containing either 0.1 μM dexamethasone (△), 0.15 μM TPA (●), or dexamethasone and TPA (▲). The cells were washed once with binding buffer and then incubated for 4 h at 4 °C with increasing concentrations of 125I IL-1 or 125I-IL-6. The binding of label to the 4 cm2 (IL-1) or 10 cm2 (IL-6) monolayer cultures was expressed according to Scatchard (55). The mean values of duplicate cultures are shown.