Fig. 5. Changes in T lymphocyte subsets after Treg infusion.

PBMC samples collected from subjects on days 0, 7, 28, and 91 were frozen from all patients then thawed simultaneously before being stained with CD4, CD45RO, CD25, CD127, CCR7, and CD38 for FACS analysis. Representative FACS plots for all analyses are shown in Supplemental Figure S6. All subjects shown are from cohort 4 (n=4), as detailed in Table 1. (A) An increase in %CD38⁺ cells was seen within CD4⁺CD45RO⁺ T cells subset post Treg infusion. (B) Within the CD4⁺CD127^lo/−CD25⁺ Tregs the percentage of CCR7⁺ Tregs increased after infusion of expanded Tregs. (C) MFI of the patients’ CD4⁺CD127^lo/−CD25⁺Treg population increased upon addition of expanded Tregs. For all plots comparisons were made to day 0 and significance was determined by paired t test.