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Three water samples were taken from the invaded commercial harbor at site #2b. DNA was isolated, and each sample was subjected to conventional PCR and touchdown PCR with or without BSA. Primers: Ponto-Caspian goby primers. Experimental control: Carp pond (site #1) sampled after the field trip. Positive control: 5 ng N. melanostomus DNA as template. Negative control: ultrapure water as template.
