. 2015 Jul 31;99(1):175–188. doi: 10.1189/jlb.3A1214-598R

TABLE 3.

Cytokines producing T cells on different time points upon MOG_35–55- induced EAE

	d 10					d 17
	WT		Ifnar1^−/−	IFNAR1^Texcl	P	WT	Ifnar1^−/−	IFNAR1^Texcl	P
dLNs
CD4⁺ IFN-γ⁺	ND		ND	ND	ND	0.64 ± 0.1	0.93 ± 0.2	0.98 ± 0.2	0.25^a
CD4⁺ IFN-γ⁺	ND		ND	ND	ND	0.64 ± 0.1	0.93 ± 0.2	0.98 ± 0.2	0.79^b
CD4⁺ IL-17⁺	ND		ND	ND	ND	0.48 ± 0.1	0.8 ± 0.2	0.6 ± 0.1	0.29^a
CD4⁺ IL-17⁺	ND		ND	ND	ND	0.48 ± 0.1	0.8 ± 0.2	0.6 ± 0.1	0.46^b
CD4⁺ IL-10⁺	ND		ND	ND	ND	0.24 ± 0.02	0.28 ± 0.04	0.32 ± 0.1	0.34^a
CD4⁺ IL-10⁺	ND		ND	ND	ND	0.24 ± 0.02	0.28 ± 0.04	0.32 ± 0.1	0.60^b
Spleen
CD4⁺ IFN-γ⁺	1.6 ± 0.2		1.8 ± 0.4	1.8 ± 0.5	0.63^a	1.75 ± 0.3	3.2 ± 0.9	3.2 ± 0.5	0.027^a
CD4⁺ IFN-γ⁺	1.6 ± 0.2		1.8 ± 0.4	1.8 ± 0.5	0.90^b	1.75 ± 0.3	3.2 ± 0.9	3.2 ± 0.5	0.97^b
CD4⁺ IL-17⁺	0.7 ± 0.1		0.8 ± 0.1	0.2 ± 0.04	0.003^a	1.62 ± 0.5	1.93 ± 0.3	1.9 ± 0.2	0.37^a
CD4⁺ IL-17⁺	0.7 ± 0.1		0.8 ± 0.1	0.2 ± 0.04	0.016^b	1.62 ± 0.5	1.93 ± 0.3	1.9 ± 0.2	0.89^b
CD4⁺ IL-10⁺	ND		ND	ND	ND	0.3 ± 0.05	0.19 ± 0.02	0.28 ± 0.03	0.59^a
CD4⁺ IL-10⁺	ND		ND	ND	ND	0.3 ± 0.05	0.19 ± 0.02	0.28 ± 0.03	0.026^b
Spinal cord
CD4⁺ IFN-γ⁺	ND	ND		ND	ND	13.44	8.7	NA^c	NA
CD4⁺ IL-17⁺	ND	ND		ND	ND	6.6	2.8	NA^c	NA

The data presented are from 2 independent experiments/time point (n = 5 mice/genotype/experiment). Measurement of cytokines in cells isolated from spinal cord was performed once. All percentages are expressed as means ± sem. The percentages represent the cytokine-positive cells gated on total live cells. ND, not determined.

Comparisons between IFNAR1^Texcl and WT mice; ^bcomparisons between IFNAR1^Texcl and Ifnar1^−/− mice; ^cvery few mononuclear cells were detected; thus, flow cytometry and statistical analysis were not applicable (NA). Boldface indicates statistically significant difference.