Table 1. Summary of the RNA sequencing data generation and alignment.

Total number of sequence reads obtained from the three cardiac chambers using RNA sequencing is described. Mapped reads refer to and include all transcripts that aligned back to the zebrafish reference genome (Zv9). The total number of uniquely expressing known protein coding genes as well as de novo transcripts are mentioned.

	Atrium	Ventricle	Bulbus arteriosus	Total (Unique)
Total reads (in millions)	210	255	270	735
Post-trimming (in millions)	198	249	263	710
Mapped reads (in millions)	162 (82%)	226 (91%)	212 (80%)	600 (84%)
Known Protein Coding Genes with expression ≥1 FPKM, RefSeq Catalog	13,105	10,567	12,404	13,396
Known Protein Coding Genes with expression ≥10 FPKM, RefSeq Catalog	5,951	6,359	5,823	7,260
De novo assembled Transcripts (Genes) with expression ≥10 FPKM	497 (352)	12,926 (8,388)	669 (442)	13,197 (8,460)