Figure 2.

Ascl2 Depletion Does Not Drive the Bulk of Gene Expression Alterations in mDKO EPCs

(A) mRNA-seq data reveal deregulated maternally controlled imprinted genes in DHet EPCs (red), whereas a paternally controlled imprinted gene is unchanged (Igf2, blue).

(B) RT-qPCR data from Ascl2 WT and mKO E7.5 EPCs shows that some key genes deregulated in mDKO EPCs are driven by Ascl2 downregulation (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; t test). Error bars represent SD.

(C) Differential expression analysis from mRNA-seq of Ascl2 mKO EPCs reveals that only a minority of Dnmt3 mDKO DE genes are explained by Ascl2 repression.

(D) Log2 fold change in expression between WT and Ascl2 or Dnmt3 mutant EPCs for each grouping of genes defined in (C). Ascl2-dependent genes display similar expression changes in Dnmt3 mDKO EPCs, whereas Dnmt3 unique DE genes are unchanged in Ascl2 mKO EPCs.

See also Figure S2.