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. 2016 Jan 25;36(2):152–163. doi: 10.1016/j.devcel.2015.12.027

Figure 3.

Figure 3

mDKO DE Genes Are Associated with Trophoblast Differentiation

(A) Several Dnmt3-specific genes affected in mDKO EPCs become upregulated during trophoblast development, as revealed by RT-qPCR of E7.5 and E9.5 WT trophoblast.

(B) Expression of mDKO DE genes in E9.5 TGCs relative to E7.5 EPCs. mRNA-seq data from E9.5 TGCs (Sher et al., 2013) were plotted against our mRNA-seq data for Ctrl (left) or mDKO (right) E7.5 EPCs. Genes upregulated in mDKO EPCs (green) have increased expression in TGCs relative to WT E7.5 EPCs. Similarly, mDKO downregulated genes (red) tend to have lower expression in TGCs. When plotted against mDKO EPC data, the expression of mDKO DE genes is much more comparable with that seen in TGCs.

(C) RT-qPCR of EMT-associated genes in E9.5 trophoblast reveals that mDKO trophoblasts do not exhibit a classic EMT phenotype, although Cdh1 is downregulated.

Error bars represent SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; t test comparing E9.5 with E7.5 (A), or ANOVA with post hoc tests comparing control (Ctrl) and mDKO genotypes (C). See also Figure S3.