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. 2015 Dec 30;5(2):227–234. doi: 10.5966/sctm.2015-0013

Figure 6.

Figure 6.

Alkaline phosphatase (ALP) activity, mineralized nodule formation, and cell viability. (A): ALP Staining. (B): Alizarin red S staining. In all cells, ALP activity and mineralized nodules became higher with time. 90D and 90O exhibited higher ALP activity and more mineral accumulation than did PD and PO. (C): Cell viability stratified by fluorescence measurement. The proliferative ability of 90D and 90O was greater than that of PD and PO after 10 days of culture. Statistically significant differences were observed between PO and 90O at day 16 (∗, p < .027; n = 5). Abbreviations: 90D, CD90(+) cells in Dulbecco’s modified Eagle’s medium; 90O, CD90(+) cells in osteogenic medium; PD, periosteum-derived cells in Dulbecco’s modified Eagle’s medium; PO, periosteum-derived cells in osteogenic medium; W, week.