Comparison of cell density, proliferation, and surface markers among AdSCs from four different adipose tissues. (A, B): MNCs were isolated from four different adipose tissues (SC, VL, CA, SS), followed by 7 days of culture to obtain adherent AdSCs. The densities in freshly isolated MNCs (A) and cultured AdSCs (B) from each source were measured and compared among the AdSCs from the four different adipose tissues. (C): The positivity of BrdU in AdSCs was assessed by immunocytochemistry. ∗, p < .05; ∗∗, p < .01; NS, not significant vs. SC. All experiments were performed in triplicate and statistically analyzed. Abbreviations: AdSCs, adipose-derived stem cells; BrdU, 5-bromo-2′-deoxyuridine; CA, cardiac brown adipose tissue; MNCs, mononuclear cells; NS, not significant; SC, subcutaneous white adipose tissue; SS, subscapular brown adipose tissue; VL, visceral white adipose tissue.