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. 2015 Dec 23;5(2):218–226. doi: 10.5966/sctm.2015-0096

Figure 2.

Figure 2.

Expression of extracellular matrix proteins in hindlimb tissues after ligation of the femoral artery. (A): Western blotting for fibronectin, laminin, collagen types I and IV, and GAPDH (control) in the nonischemic and ischemic legs of mice before surgery and 1, 3, 7, 14 and 28 days after surgery. (B): Immunofluorescence showing strong expression of fibronectin (red) in the ischemic hindlimbs at the chronic stage after femoral artery ligation (28 days after surgery). Scale bars = 50 μm. Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; I, ischemic; NI, nonischemic; Pre, preoperatively.