Figure 6.
Vascular immunofluorescence of the ischemic hindlimb of a mouse systemically treated with ECFCs overexpressing integrin β1. (A): Origin of the detected endothelial cells. Detection of total vessels using Lycopersicon esculentum lectin and human vessels (arrows) using anti-human Flk-1 antibody. Scale bars = 50 μm. (B): Testing for cell fusion by double immunostaining for human- and mouse-derived endothelial cells using anti-human Flk-1 (cross-reacting with human but not with mouse endothelial cells) and anti-mouse CD31 (cross-reacting with mouse but not with human endothelial cells) antibodies. No detected endothelial cells were positive for both antibodies, indicating no cell fusion. Scale bar = 50 μm.